Examining the effects of a multicomponent oral antioxidant therapy on semen parameters and seminal oxidative stress in males with idiopathic infertility

Document Type : Original Article

Authors

1 Dermatology department , Faculty of Medicine, Beni-Suef University, Egypt

2 Andrology, Sexology and STDs department, Faculty of Medicine, cairo University, Egypt

3 Andrology, Sexology and STDs department, Faculty of Medicine, Beni-Suef University, Egypt

Abstract

The aim of this study to assess the efficacy of a ten constituent oral antioxidant formula, on various semen parameters and seminal oxidative stress in males with idiopathic infertility. Single center, prospective, comparison the efficacy of a multicomponent oral antioxidant drug and a matching placebo, Thirty infertile males were included and subjected to detailed history taking, complete general and genital examination, conventional semen analysis, seminal oxidative stress assay by the MiOXSYS system and finally receiving an oral antioxidant for one month followed by one month washout period, then a seemingly identical placebo was given.

Keywords

Main Subjects


1- Catanzariti F, Cantoro U, Lacetera V, Muzzonigro G, Polito M. (2013). Comparison between WHO (World Health Organization) 2010 and WHO 1999 parameters for semen analysis – interpretation of 529 consecutive samples. Arch Ital Urol Androl. 2013; 85(3): 125–9.
2- Esteves, S. C. (2014). Clinical relevance of routine semen analysis and controversies surrounding the 2010 World Health Organization criteria for semen examination. International braz j urol, 40(4), 433-453.
3- Agarwal, A., Durairajanayagam, D., & Du Plessis, S. S. (2014). Utility of antioxidants during assisted reproductive techniques: an evidence based review. Reproductive Biology and Endocrinology, 12(1), 112.
4- Agarwal, A., & Bui, A. D. (2017). Oxidation-reduction potential as a new marker for oxidative stress: Correlation to male infertility. Investigative and clinical urology, 58(6), 385-399.
5- Agarwal, A., Gupta, S., & Sharma, R. (2016).Reactive oxygen species (ROS) measurement. In andrological evaluation of male infertility (pp. 155-163). Springer, Cham.
6- Zini, A., San Gabriel, M., &Baazeem, A. (2009). Antioxidants and sperm DNA damage: a clinical perspective. Journal of Assisted Reproduction and Genetics, 26(8), 427-432.
7- Haghighian H, Haidari F, Mohammadi-Asl J, Dadfar M. Randomized, tripleblind, placebo-controlled clinical trial examining the effects of alpha-lipoic acid supplement on the spermatogram and seminal oxidative stress in infertile men. FertilSteril 2015; 104: 318–324.
8- Gvozdjáková, A., Kucharská, J., Dubravicky, J., Mojto, V., & Singh, R. B. (2015). Coenzyme Q10, α-tocopherol, and oxidative stress could be important metabolic biomarkers of male infertility. Disease markers, 2015.
9- Agarwal, A., Gupta, S., & Sharma, R. (2016).Reactive oxygen species (ROS) measurement. In andrological evaluation of male infertility (pp. 155-163). Springer, Cham.
10- World Health Organization.(2010). WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge university press.
11- Agarwal, A., Gupta, S., & Sharma, R. (2016).Reactive oxygen species (ROS) measurement. In andrological evaluation of male infertility (pp. 155-163). Springer, Cham.
12- Chan YH (2003a): Biostatistics102: Quantitative Data – Parametric & Non-parametric Tests. Singapore Med J.;44(8): 391-396 .
13- Chan YH (2003b): Biostatistics 103: Qualitative Data –Tests of Independence. Singapore Med J.;44(10): 498-503 .
14- Chan YH (2003c): Biostatistics 104: Correlational Analysis. Singapore Med J.;44(12) : 614-619.
15- Zini, A., San Gabriel, M., &Baazeem, A. (2009). Antioxidants and sperm DNA damage: a clinical perspective. Journal of Assisted Reproduction and Genetics, 26(8), 427-432.
16- Alahmar, A. T. (2019). Role of oxidative stress in male infertility: An updated review. Journal of human reproductive sciences, 12(1), 4.
17- Tremellen, K. (2008). Oxidative stress and male infertility—a clinical perspective. Human reproduction update, 14(3), 243-258.
18- Balercia, G., Regoli, F., Armeni, T. A., Koverech, A., Mantero, F., &Boscaro, M. (2014). Placebo-controlled double-blind randomized trial on the use of L-carnitine, L-acetylcarnitine, or combined L-carnitine and L-acetylcarnitine in men with idiopathic asthenozoospermia. Andrologiâ i GenitalʹnaâHirurgiâ, 15(1), 59-68.
19- Alahmar, A. T. (2019). Role of oxidative stress in male infertility: An updated review. Journal of human reproductive sciences, 12(1), 4.
20- Alahmar, A. T. (2019). Role of oxidative stress in male infertility: An updated review. Journal of human reproductive sciences, 12(1), 4.
21- Wirleitner, B., Vanderzwalmen, P., Stecher, A., Spitzer, D., Schuff, M., Schwerda, D., ...& Herbert Zech, N. (2012). Dietary supplementation of antioxidants improves semen quality of IVF patients in terms of motility, sperm count, and nuclear vacuolization. Int J VitamNutr Res, 82(6), 391-8.
22- Safarinejad, M. R. (2012). The effect of coenzyme Q 10 supplementation on partner pregnancy rate in infertile men with idiopathic oligoasthenoteratozoospermia: an open-label prospective study. International urology and nephrology, 44(3), 689-700.
23- Busetto, G. M., Koverech, A., Messano, M., Antonini, G., De Berardinis, E., & Gentile, V. (2012).Prospective open-label study on the efficacy and tolerability of a combination of nutritional supplements in primary infertile patients with idiopathic astenoteratozoospermia. Arch ItalUrolAndrol, 84(3), 137-40.
24- Agarwal, A., Tvrda, E., & Sharma, R. (2014).Relationship amongst teratozoospermia, seminal oxidative stress and male infertility. Reproductive Biology and Endocrinology, 12(1), 45.
25- Bozhedomov, V. A., Lipatova, N. A., Bozhedomova, G. E., Rokhlikov, I. M., Shcherbakova, E. V., &Komarina, R. A. (2017). Using L-and acetyl-L-carnintines in combination with clomiphene citrate and antioxidant complex for treating idiopathic male infertility: a prospective randomized trial.Urologiia (Moscow, Russia: 1999), (3), 22-32.
26- ImamovicKumalic, S., & Pinter, B. (2014). Review of clinical trials on effects of oral antioxidants on basic semen and other parameters in idiopathic oligoasthenoteratozoospermia. BioMed research international, 2014.
27- ImamovicKumalic, S., & Pinter, B. (2014). Review of clinical trials on effects of oral antioxidants on basic semen and other parameters in idiopathic oligoasthenoteratozoospermia. BioMed research international, 2014.
28- Wirleitner, B., Vanderzwalmen, P., Stecher, A., Spitzer, D., Schuff, M., Schwerda, D., ...& Herbert Zech, N. (2012). Dietary supplementation of antioxidants improves semen quality of IVF patients in terms of motility, sperm count, and nuclear vacuolization. Int J VitamNutr Res, 82(6), 391-8.
29- Wirleitner, B., Vanderzwalmen, P., Stecher, A., Spitzer, D., Schuff, M., Schwerda, D., ...& Herbert Zech, N. (2012). Dietary supplementation of antioxidants improves semen quality of IVF patients in terms of motility, sperm count, and nuclear vacuolization. Int J VitamNutr Res, 82(6), 391-8.
30- Comhaire, F. H., Christophe, A. B., Zalata, A. A., Dhooge, W. S., Mahmoud, A. M. A., &Depuydt, C. E. (2000). The effects of combined conventional treatment, oral antioxidants and essential fatty acids on sperm biology in subfertile men. Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA), 63(3), 159-165.
31- World Health Organization.(2010). WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge university press.
32- Ghanem, H., Shaeer, O., & El-Segini, A. (2010). Combination clomiphene citrate and antioxidant therapy for idiopathic male infertility: a randomized controlled trial. Fertility and sterility, 93(7), 2232-2235.
33- Wirleitner, B., Vanderzwalmen, P., Stecher, A., Spitzer, D., Schuff, M., Schwerda, D., ...& Herbert Zech, N. (2012). Dietary supplementation of antioxidants improves semen quality of IVF patients in terms of motility, sperm count, and nuclear vacuolization. Int J VitamNutr Res, 82(6), 391-8.
34- Abad, C., Amengual, M. J., Gosalvez, J., Coward, K., Hannaoui, N., Benet, J., &Prats, J.(2013). Effects of oral antioxidant treatment upon the dynamics of human sperm DNA fragmentation and subpopulations of sperm with highly degraded DNA. Andrologia, 45(3), 211-216.
35- World Health Organisation.(1999). WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. Cambridge university press.
36- Danis, R. B., &Samplaski, M. K. (2019). Sperm Morphology: History, Challenges, and Impact on Natural and Assisted Fertility. Current urology reports, 20(8), 43.
37- Cavallini, G. (2006). Male idiopathic oligoasthenoteratozoospermia. Asian journal of andrology, 8(2), 143-157.
38- Safarinejad, M. R., Safarinejad, S., Shafiei, N., &Safarinejad, S. (2012). Effects of the reduced form of coenzyme Q10 (ubiquinol) on semen parameters in men with idiopathic infertility: a double-blind, placebo controlled, randomized study. The Journal of urology, 188(2), 526-531.
39-Busetto, G. M., Koverech, A., Messano, M., Antonini, G., De Berardinis, E., & Gentile, V. (2012).Prospective open-label study on the efficacy and tolerability of a combination of nutritional supplements in primary infertile patients with idiopathic astenoteratozoospermia. Arch ItalUrolAndrol, 84(3), 137-40.
40- Bozhedomov, V. A., Lipatova, N. A., Bozhedomova, G. E., Rokhlikov, I. M., Shcherbakova, E. V., &Komarina, R. A. (2017). Using L-and acetyl-L-carnintines in combination with clomiphene citrate and antioxidant complex for treating idiopathic male infertility: a prospective randomized trial.Urologiia (Moscow, Russia: 1999), (3), 22-32.